A fluorescent probe can spot the difference between the four DNA bases: adenine, thymine, cytosine and guanine.rnSnorri Sigurdsson and Pavol Cekan from the Universty of Iceland, Reykjavik, have synthesised a highly fluorescent nucleoside (a base bound to a sugar) which can report the identity of its base-pair when placed in a DNA duplex. The fluorescence emitted by the duplex differs depending on which DNA base is paired with the probe.rnAccording to Sigurdsson and Cekan, the probe could find use in detecting single DNA base changes - single nucleotide polymorphisms (SNP) - at sites where variation can indicate disease. Changes within the gene apolipoprotein E, for example, are considered to be an indicator of predisposition to Alzheimer's. The researchers explain that detecting SNPs with their probe could makernit possible to identify genetic diseases or recognise people who might be susceptible to them. Also, the probe would not only detect a SNP but could indicate which DNA base replaced the correct one.
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机译:6.一种化合物,其包含具有根据20的EP-B1-2563920(SEQ ID NO:80)的具有由20个连接的核苷组成的核碱基序列的修饰的寡核苷酸,其中所述修饰的寡核苷酸包含:由十个连接的脱氧核苷组成的缺口片段;和由五个连接的核苷组成的5'翼区段; 3'翼段由五个连接的核苷组成;其中所述间隙片段位于5'侧翼片段和3'侧翼片段之间,其中每个侧翼片段的每个核苷包含2'-O-甲氧基乙基糖;其中修饰的寡核苷酸的每个胞嘧啶是5-甲基胞嘧啶,并且其中修饰的寡核苷酸的每个核苷间键是硫代磷酸酯键;特别是inotersen;及其衍生物,例如由EP-B1-2563920保护的其盐,包括钠盐。