Isolation of calcium phosphate crystals from complex biological fluids using bisphosphonate-modified superparamagnetic beads

摘要

The surface of superparamagnetic magnetic beads was modified with bisphosphonates to selectively capture calcium phosphate crystals from complex biological fluids (i.e. synovial fluid).rnCalcium phosphate crystals such as hydroxyapatite (HA), basic calcium phosphate (BCP) and calcium pyrophosphate dihydrate (CPPD) are known to occur in intra-articular synovial fluid and may be linked to a number of destructive joint conditions. For instance, BCP crystals are found in up to 60% of synovial fluid samples from patients with knee joint osteoarthritis (OA) and CPPD crystal deposition is observed in a variety of clinical manifestations including acute pseudog-out, which suggest that calcium phosphate crystals may represent a potential therapeutic target. Progress in understanding the role of calcium crystals in different disease states is hindered by our limited ability to detect them accurately. Hence, the development of potential therapies is restricted.