Immobilized enzyme-single-wall carbon nanotube composites for amperometric glucose detection at a very low applied potential

摘要

The behaviour of support electrodes modified with randomly dispersed single-wall carbon nanotube meshes containing adsorbed glucose oxidase with respect to amperometric glucose detection at a low potential is demonstrated. Electron transfer in biological systems is one of the leading areas in the biochemical and biophysical sciences, and in recent years there has been considerable interest in the direct electron transfer between redox proteins and electrode surfaces. However in the absence of mediating small molecules, the observation of well defined electrochemical behaviour of immobilized flavoprotein oxidase systems such as Glucose Oxidase (GOx) is rendered extremely difficult, because the . FAD group is embedded deep within the protein structure thereby making the transmission coefficient for direct electron transfer between the latter and a support electrode very small. Various immobilization strategies have been adopted to fabricate enzyme electrodes for biosensor applications.