Ligand design for the improvement of stability of metal complex·protein hybrids

摘要

We have succeeded in improving the stability of Fe(Schiff-base)·heme oxygenase (HO) hybrids by ligand design based on the crystal structure of Fe(N,N'-bis(salicylidene)-3.4-diamino-benzene propionic acid)·HO. Hybridization of synthetic metal complexes and proteins is an attractive target for the preparation of catalysts, sensors, and metal drugs. Non-covalent interactions between metal cofactors and surrounding amino acid residues are very important to accommodate cofactors in the protein cavity as evidenced by the crystal structures of composites.