In vivo two-photon fluorescent imaging of fluoride with a desilylation-based reactive probe

摘要

A two-photon excitable molecular probe for fluoride, developed based on a fluoride-specific desilylation reaction, is demonstrated to be useful for fluorescent imaging of fluoride ions in live zebraflsh by one-photon as well as two-photon microscopy for the first time. Fluoride is added to drinking water to promote dental health in some countries (water fluoridation). There is consistent evidence that water fluoridation, however, results in the development of dental fluorosis.