METHOD OF SIMULTANEOUSLY IMAGING SODIUM/CALCIUM ACTIVITIES USING TWO-PHOTON FLUORESCENT PROBE OF MUTUALLY DIFFERENT FLUORESCENT COLORS AND PREPARATION METHOD OF TWO-PHOTON FLUORESCENT PROBE

摘要

PROBLEM TO BE SOLVED: To provide a method of simultaneously imaging sodium/calcium activities using a two-photon fluorescent probe of mutually different fluorescent colors, and a method for preparing the two-photon fluorescent probe.;SOLUTION: The disclosed two-photon fluorescent probe for calcium ions near a cell membrane reacts with calcium cations to exhibit strong two-photon fluorescence and may be selectively and easily loaded into the cell membrane by forming a complex with a calcium ion. Also, it allows imaging of the distribution of calcium cations in a living cell or tissue since it can selectively detect calcium ions in the living cell or living tissue at a depth of 100 to 200 m for 60 minutes or more. In addition, by simultaneously staining the living cell or tissue with two probes of different fluorescent colors, the calcium and sodium activities can be imaged at different channels.;COPYRIGHT: (C)2012,JPO&INPIT