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DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles

机译:横向流动试纸条上的DNA检测:使用LNA共轭金纳米颗粒增强信号灵敏度

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摘要

A lateral flow test strip assay, enabling sensitive detection of DNA specific to the foodborne pathogen E. coli O157:H7, is described. The use of LNA-conjugated gold nanoparticle probes, along with signal amplification protocols, results in minimum detectable concentrations of ~ 0.4 nM.
机译:描述了一种侧向流动试纸测定法,其能够灵敏地检测到食源性病原体大肠杆菌O157:H7特异的DNA。 LNA共轭金纳米粒子探针的使用,以及信号放大方案,可导致的最低可检测浓度约为0.4 nM。

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  • 来源
    《Chemical Communications》 |2012年第62期|p.7714-7716|共3页
  • 作者单位

    Biosensors and Nanotechnology Applications Laboratory,University of Idaho, Coeur d'Alene, ID 83814-2277, USA,Department of Chemistry, University of Idaho, Moscow, ID 83844-2343, USA;

    Biosensors and Nanotechnology Applications Laboratory,University of Idaho, Coeur d'Alene, ID 83814-2277, USA;

    Biosensors and Nanotechnology Applications Laboratory,University of Idaho, Coeur d'Alene, ID 83814-2277, USA;

    Department of Chemical and Materials Engineering,University of Idaho, Moscow, ID 83844-1021, USA;

    Biosensors and Nanotechnology Applications Laboratory,University of Idaho, Coeur d'Alene, ID 83814-2277, USA;

    Department of Chemistry, University of Idaho, Moscow, ID 83844-2343, USA;

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  • 入库时间 2022-08-17 13:20:56

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