首页> 外国专利> STRIP FOR HIGH-SENSITIVITY LATERAL FLOW IMMUNOASSAY BASED ON SURFACE-ENHANCED RAMAN SCATTERING AND DETECTION METHOD USING SAME

STRIP FOR HIGH-SENSITIVITY LATERAL FLOW IMMUNOASSAY BASED ON SURFACE-ENHANCED RAMAN SCATTERING AND DETECTION METHOD USING SAME

机译:基于表面增强拉曼散射检测的高灵敏度横向流动免疫检测技术

摘要

The present invention relates to a surface-enhanced Raman scattering (SERS) lateral flow immunoassay strip comprising: a sample pad, into which a sample comprising a target material is introduced; a conjugate pad comprising a hollow metal nanoprobe for surface-enhanced Raman scattering, on which an antibody that can be coupled to the target material and a Raman marker are immobilized; and a detection pad comprising a detection area, to which a secondary antibody is fixed, the second antibody being capable of being coupled to the target material coupled to the hollow metal nanoprobe. Use of the SERS-based lateral flow immunoassay strip according to the present invention simultaneously enables high-sensitivity quantitative analysis and qualitative analysis from Raman signal measurement that follows the concentration of the target material.
机译:本发明涉及一种表面增强拉曼散射(SERS)侧向流免疫测定条,其包括:样品垫,其中引入了包含靶材料的样品;以及缀合物垫,其包含用于表面增强拉曼散射的中空金属纳米探针,其上固定有可偶联至靶标材料的抗体和拉曼标记物;所述检测垫包括检测区域,所述检测区域固定有第二抗体,所述第二抗体能够与与所述中空金属纳米探针偶联的靶材料偶联。同时使用根据本发明的基于SERS的侧向流免疫测定条,能够根据跟随目标物质浓度的拉曼信号测量进行高灵敏度的定量分析和定性分析。

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