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首页> 外文期刊>Chemical Communications >Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides
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Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides

机译：迈向高度灵活的适体：未锁定核酸核苷酸的酶识别研究

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摘要

Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modifled DNA library was efficiently achieved by KOD DNA polymerase.
机译：已成功完成对解锁核酸（UNA）核苷酸的酶识别。发现热解DNA聚合酶是引物延伸反应中的有效酶。 KOD DNA聚合酶可有效实现81 mer UNA修饰的DNA文库的聚合酶链反应（PCR）扩增。

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  • 来源
    《Chemical Communications》 |2012年第44期|p.5503-5505|共3页
  • 作者

    Camille Dubois; Meghan A. Campbell; Stacey L. Edwards; Jesper Wengel; Rakesh N. Veedu;

  • 作者单位

    School of Chemistry and Molecular Biosciences,The University of Queensland, St Lucia, Brisbane, 4072, Australia;

    Nucleic Acid Center, Department of Physics and Chemistry,University of Southern Denmark, Campusvej 55, Odense M, 5230,Denmark;

    School of Chemistry and Molecular Biosciences,The University of Queensland, St Lucia, Brisbane, 4072, Australia;

    Nucleic Acid Center, Department of Physics and Chemistry,University of Southern Denmark, Campusvej 55, Odense M, 5230,Denmark;

    School of Chemistry and Molecular Biosciences,The University of Queensland, St Lucia, Brisbane, 4072, Australia;

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