Mito-DEPMPO synthesized from a novel NH_2-reactive DEPMPO spin trap: a new and improved trap for the detection of superoxide

摘要

Mito-DEPMPO, a new DEPMPO analogue bearing a triphenylphosphonium group, was synthesized via a novel NH_2-reactive DEPMPO. The half-life of the Mito-DEPMPO superoxide adduct was estimated to be ca. 40 min. Using Mito-DEPMPO, reactive oxygen species generated in intact mitochondria were detected and characterized by EPR.