Determination of Trenbolone Residual in Bovine Liver by Liquid Chromatography–Mass Spectrometry

摘要

A sensitive method was developed and validated for the quantitation of trenbolone residues in bovine liver. Target analytes were extracted from liver homogenate using solid phase cartridges, separated on a Phenyl column and detected using an electrospray ionization mass spectrometer operating in positive ion mode. The mean recovery of the analytes was between 62% and 69%. The method provided detection capabilities of 1 μg/kg for trenbolone. The method is suitable for application in veterinary drug residue in surveillance programme. Keywords Trenbolone - LC–ESI–MS - Bovine liver - Residue