Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment: a radioimmuno- conjugate for hepatocellular carcinoma imaging

摘要

AIM To directly radiolabel an anti-hepatoma mAb fragment Hab18 F (ab')_2 with 99m Tc by stannous-reduced method, and assess the stability, biodistribution and radioimmun- oimaging (RII). METHODS Immunoreactive fraction was Determined according to Lindmo's method. Ellman's reagent was used to determine the Number of thiols in the reduced F9ab)_2. Labeling Efficiency and homogeneity were measured by Paper chromatography, sodium dodecylsulphate Polyacrylamide gel electrophoresis 9SDS-PAGE) And autoradiography.