Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates

摘要

AIM To develop a culture model providing durable biomaterials with high yields and activities used in bioartificial liver. METHODS Hepatocytes were isolated from a Whole pig liver by Seglen's method of orthotopic Prfusion with collagenase. In culture on Microcarriers, primary porcine hepatocytes Were inoculated at a concentration of 5 × 10~7 /mL Into the static culture systems containing 2 g/L Cytodex-3, then supplemented with 100 Ml/l Fetal calf serum (FCS) or 100 mL/L porcine Portal vein serum (PPVS) respectively.