Differential expression of a novel colorectal cancer differentiation-related gene in colorectal cancer

摘要

AIM To investigate SBA2 expression in CRC cell lines and surgical specimens of CRC and autologous healthy mucosa. METHODS Reverse transcription-polymerase chain METHODS Reverse transcription-polymerase chain Reaction (RT-PCR) was used for relative quantification of SBA2 mRNA levels in 4 human CRC cell lines with different Grades of differentiation and 30 clinical samples. Normalization of the results was achieved by simultaneous Amplification of β-actin as an internal control. RESULTS In the exponential range of amplification, fairly Good linearity demonstrated identical amplification Efficiency for SBA2 and β-actin (82/100).