Influencing factors of rat small intestinal epithelial cell cultivation and effects of radiation on cell proliferation

摘要

Crypt epithelial cells in normal small intestine proliferate at a high speed. But they are very difficult to culture in vitro and passage stably. A lot of studies have been done ~[1-16]. Some domestic labs isolated and cultured crypt cells from embryonal intestines and aseptic animal intestine, but failed. We introduced normal rat epithelial cell line-IEC-6 From the USA and its living condition for stable Passage was successfully established after trials. The Cell line was testified to be the small intestinal; Epithelial cell by electron microscopy, Immunihistochemistry and enzymatic histoch- Emistry. It has been applied to some related Research work~[17-21]. It was found that many Factors were involved in the culture system. Our Present study focuses on the culture method and the Influencing factors on IEC-6.