Inhibition of nitric oxide synthase abrogates lipopolysaccharides-induced up-regulation of L-arginine uptake in rat alveolar macrophages

摘要

It was tested whether the inducible nitric oxide(iNOS)pathway might be involved in lipopolysaccharides-(LPS)-induced up-regulation of L-arginine transport in rat alveolar macrophages (AMφ). AMφwere cultured in absence or presence of LPS. Nitrite accumulation was determined in Culture media and cells were used to study[~3H]-L-arginine uptake or to isolate RNA for RT-PCR. Culture in presence of LPS(1μg ml~-1, 20 h)caused 11 fold increase of nitric accumulation and 2.5 fold increase of[~3H]-L-arginine uptake.