β_2-adrenoceptor agonists inhibit release of eosinophil-activating cytokines from human airway smooth muscle cells

摘要

Airway smooth muscle(ASM)is a potential source of multiple pro-inflammatory cytokines during airway inflammation.β-Adrenoceptor agonist hyporesponsiveness is a characteristic feature of asthma, and interleukin(IL)-1βand tumour necrosis factor(TNF)-αare implicated in its cause. Here, the capacity ofβ-adrenoceptor agonists to prevent release of GM-CSF, RANTES, eotaxin and IL-8, elicited by IL-1βor TNF-α, was examined in human ASM cells. Isoprenaline(～EC_50 150 nM), a non-selectiveβ-adrenoceptor agonist, and salbutnmol(～EC_50 25 nM), a selectiveβ_2-adrenoceptor agonist, attenuated release of GM-CSF, RANTES and eotaxin, but not IL-8(EC_50 > 1μM).