Short- and long-term changes in blood miRNA levels after nanogold injection in rats—potential biomarkers of nanoparticle exposure

摘要

Context: Increased use of engineered nanoparticles may result in exposure of workers and consumers, making them a health concern.Objective:To identify potential blood miRNA biomarkers after intravenous gold nanoparticle (AuNP) exposure. Materials and methods: miRNA microarray analysis was carried out on blood of rats at 1 week and 2 months after injection.Results: Many up- and downregulated miRNAs were detected. Of these, rno-miR-298 was confirmed to be increased at 1 week postinjection by reverse transcription-PCR (RT-PCR).Discussion and conclusion: Blood miRNAs could be useful as biomarkers for exposure to nanoparticles. miR-298 regulates p-amyloid (Ap) precursor protein-converting enzyme-1 (BACE1) in Alzheimer's disease.