Overexpression of kin17 protein forms intranuclear foci in mammalian cells

摘要

We used antibodies against E coli RecA protein to identify in mouse cells a 45-kDa DNA-binding protein called kin17, which has an active zinc finger and a nuclear localization signal. Kin17 protein produced in E coli binds preferentially to the curved DNA of a bacterial promoter in viro and in vitro, suggesting a transcriptional regulation activity. The fact that in rodent cells kin17 protein levels increase after gamma-irradiation suggests its participation in a cellular response to ionising radiation.