Identification of ribosomal protein S1 as a poly(A) binding protein in Escherichia coli

摘要

To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli, we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot lechnique, a protein in the ribosomal supernatant fraction of E coli was identified and purified to homogeneity. N-terminal sequence analysis yielded a 25-residue sequence which corresponded to the 25 N-terminal amino acids of protein S1, one of the proteins of the E coli 30S ribosomal subunit.