Pseudomonas rhodesiae PF1: A New and Efficient Biocatalyst for Production of Isonovalal from a-pinene Oxide

摘要

A new bacterial strain, identified as Pseudomonas rhodesiae PF1 and deposited under the accession number CIP 107491, is presented. It is very active for the production of the acyclic compound Z-2-methyl-5-isopropyl-hexa-2,5-dien-l-al (isonovalal) from a-pinene oxide. Enzyme synthesis is induced by culturing cells on a-pinene; growing bacteria also have the ability to synthesize the epoxide derivative of a-pinene. Isonovalal production was performed without aeration and with concentrated resting cells previously frozen at -20℃, and subsequently thawed in a water-organic solvent, two-phase system. The organic layer was hexadecane, the volume ratio being 1:1. The best results achieved allowed recovery of c.a. 60 g/l organic solvent of isonovalal in 2.5 h operation, which is the most efficient process to date in the area of terpene biotransf ormations.