Validation of reference genes for real-time quantitative polymerase chain reaction analysis in Lactobacillus plantarum R23 under sulfur dioxide stress conditions

摘要

Background and AimsMalolactic fermentation (MLF), accomplished by lactic acid bacteria (LAB), is indispensable for the production of some red wines. As the key additive for wines, sulfur dioxide (SO2) frequently affects LAB growth and MLF development. We aimed to investigate suitable reference genes in Lactobacillus plantarum R23, an excellent LAB with good tolerance to SO2, for a real-time quantitative PCR analysis of SO2 tolerance.