Determination of proper treatment time for in vivo blood coagulation and wound healing application by non-thermal helium plasma jet

摘要

The aim of this research was to determine the time of blood coagulation of in vivo cuts that treated by the non-thermal atmospheric pressure plasma jet. Also, the effect of different treatment times on wound healing has been studied. The non-thermal atmospheric pressure plasma jet working in helium gas has been used. The averaged treatment time of 8.6s for in vivo cuts on the Balb/c mouse liver showed the complete blood coagulation. Also, the effect of tretament time on wound healing has been studied by applying plasma on the wounds for different times (10, 20, 30, 40 and 50s). It was obtained from morphological analysis that the treatment groups of 30s, 40s and 50s cause the wounds to be healed faster than the groups 10s and 20s.The histological analysis showed that in 30s and 40s treatment time groups, the repair process of treated wounds has been accelerated, while for 50s group, it has not been completed, yet. The 30s treatment time has been chosen because of imposing lower dose to living tissue. The treated wound area reduction ratios against the control wound reduction ratios for 30s group were obtained 78%, 77% and 63% at the 3rd, 5th and 8th days, respectively.