Full-genome sequence and analysis of a novel human rhinovirus strain within a divergent HRV-A clade

摘要

Genome sequences of human rhinoviruses (HRV) have primarily been from stocks collected in the 1960s, with genomes and phylogeny of modern HRVs remaining undefined. Here, two modern isolates (hrv-A101 and hrv-A101-v1) collected ~8 years apart were sequenced in their entirety. Incorporation into our full-genome HRV alignment with subsequent phylogenetic network inference indicated that these represent a unique HRV-A, localized within a distinct divergent clade. They appear to have resulted from recombination of the hrv-65 and hrv-78 lineages. These results support our contention that there are unrecognized distinct HRV-A strains, and that recombination is evident in currently circulating strains. Nucleotide sequence data reported are available in the GenBank databases under the accession numbers GQ415051 and GQ415052 for hrv-A101 and hrv-A101-v1, respectively.