Synthesis of Biogenic Chitosan Biopolymer‑Functionalized Zinc‑Doped Bi_2O_3 Nanoneedles and Its Bio‑applications: In Vitro Antibacterial and Anticancer activity

摘要

A new biogenic chitosan biopolymer-functionalized zinc-doped bismuth oxide nanoneedle was successfully synthesized by an ultrasound-assisted chemical precipitation method that annealed at 250°C. In this process, an appropriate amount of chitosan, Bi (NO_3)_2, sodium hydroxide and varying molar ratios of Zn (NO_3)_2 were used as the source materials. The obtained materials were subjected to annealing at 250°C and then characterized with X-ray diffraction, Fourier transform infrared spectroscopy, high-resolution scanning electron microscope, transmittance electron microscope, and UV–Vis–NIR spectroscopy (UV–Vis–NIR). The electrochemical properties of the materials were investigated by electrochemical impedance spectroscopy, and cyclic voltammetry in the pH range is 4 with (0.1) M buffer solution. Finally, the potential toxicity and antibacterial activity of the materials were investigated against Staphylococcus aureus, Escherichia coli and cancer cell lines HepG2 and C3A. The result indicates that CS/Zn_(0.75)Bi_2O_3 nanoneedle shows zones of inhibition against S. aureus as 32 mm and E. coli as 35 mm. In the anticancer analysis, CS/Zn_(0.75)Bi_2O_3 nanoneedles showed a maximum cell inhibition of 65.45% at 100 μg/mL~(−1).