Semi-continuous mass culture of rotifers (Brachionus plicatilis) using an automatic feeder

摘要

This study describes the rotifer mass culture protocol developed at the Institute of Aquaculture (HCMR) in Crete. Rotifers were cultured semi-continuously at a density of 257.6 ± 2.6?ind.?mL?1, with a daily dilution rate of 25%. An automatic feeder was used to administer dry feed (Selco Sparkle?) every 10?min. A number of factors were investigated so as to provide an in-depth analysis of the advantages and disadvantages of the two systems, especially when compared with traditional protocols such as batch culture. The use of these two systems resulted in a culture of considerable duration (41 days) and daily yield (0.21 ± 0.01?ind.?106?day?1?L?1), using exclusively dry feed. Both systems can be easily applied in hatcheries, requiring few modifications on existing protocols and contribute to a stable and predictable production. Further improvements include manipulation of the dilution rate, food ration, culture volume and duration, as well as choice of a phytoplankton-based diet.