Electrochemical detection of amikacin sulphate using reduced graphene oxide and silver nanoparticles nanocomposite

摘要

This work examined the electrochemical detection of amikacin sulphate by utilizing a nano composite of green synthesized silver nanoparticles and reduced graphene oxide (AgNPs/rGO) on a nickel foam (NiF) electrode. The average size of AgNPs was 35 nm. The transmission electron microscopic and field emission scanning electron microscopic images of the rGO-AgNPs composite confirmed that the AgNPs stiffly adhered to the rGO sheets. The cyclic voltammetry (CV) and differential pulse voltammograms (DPVs) were employed for the characterization of electrodes. The electrochemical behavior of the (NiF-AgNPs/rGO) electrode and the surface confined oxidation of amikacin sulphate were confirmed by CV. The developed sensor showed a linear electrochemical response in the range of 0.05-15 mu M with a detection limit of 38 nM. The developed sensor exhibited reproducibility with a relative standard deviation of 1.01% and storage stability over four weeks. The results revealed that owing to the uniform distribution of AgNPs on rGO; the fabricated sensor had high selectivity and response to amikacin sulphate detection. Moreover, the practical viability of the developed sensor was evaluated in spiked human urine samples under validated conditions. The recovery rate was in the range of 99%-102%, which indicates the sensor's effectiveness in the detection of amikacin sulphate.