Disposable Amperometric A-fetoprotein Immunosensor Based on the Biocompatible Silk Protein Membranes-Modified Indium Tin Oxide Electrodes

摘要

A simple and disposable electrochemical immunosensor for detection of 68Â kDa alpha-fetoprotein (AFP) was fabricated based on films of silk fibroin protein membrane (SFPM)/Prussian blue (PB)/deposition of gold nanoparticles (DpAu). First, DpAu and PB were electrochemically deposited successively on the surface of indium tin oxide (ITO) electrode. Then, SFPM with excellent biocompatibility was modified on the surface of PB/DpAu/ITO. The SFPM could form a stable matrix on the electrode surface for the deposition of immunoactive agents. More importantly, the SFPM could prevent the possible leakage of electron mediator and enhance the stability of immunosensor. Scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize the assembly process of the modified electrode. The linear range of the proposed immunosensor extended from 1.0 to 200.0Â ng/mL for detection of AFP with a detection limit of 0.6Â ng/mL. Moreover, the CV test demonstrated the immunosensor exhibited acceptable reproducibility and stability. This composite membrane could be applied for the detection of different biomarkers, diagnosis, and monitoring of carcinoma. [Supplementary materials are available for this article. Go to the publisher's online edition of Analytical Letters for the following free supplemental resources: additional figures.] View full textDownload full textKeywordsAlpha-fetoprotein (AFP), Deposition of gold nanoparticles (DPAU), Indium tin oxide (ITO), Prussian blue (PB), Silk fibroin protein membrane (SFPM)Related var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/00032719.2011.653898