ポリビニルアルコールゲルを用いるゲル浸透クロマトグラフィーにおけるポリ塩化ビフェニルと絶縡油の分離メカニズム

摘要

ポリ塩化ビフェニル（PCB）迅速分析に有効なクリーンアップ剤であるポリビニルアルコール（PVA）ゲrnル上でのPCBと絶縁油の分離メカニズムの解明を試みた．アセトン移動相における，脂肪族炭化水素，アrnルキルベンゼン，多環芳香族炭化水素，及びこれらの各塩素化物の溶出位置を確認した．これより，絶縁油rnはサイズ排除作用，PCBはサイズ排除作用と分配作用が働いており，検討した化合物の溶出位置と，有機rn概念図の指標として用いられる無機性/有機性との間に良好な比例関係が確認された．これらの関係から，rnPVAゲル上では無機性/有機性が大きなPCBでは分配作用が強く働くため，絶縁油との分離が達成されるrnことが分かった．この分離メカニズムは．van't Hoffプロットから求めた熱力学量（△H）から説明するこrnとができ，PVAゲル上の分離特性は，無機性/有機性及び熱力学量を用いて評価することが可能となった．%We studied the separation mechanism of polychlorinated biphenyls (PCB) from insulating oil by using an acetone mobile-phase on polyvinyl alcohol (PVA) gel, which is used as a high-performance PCB clean-up material for gel permeation chromatography. Based on the results of measurements on each elution characteristic for various organic compounds, such as aliphatic hydrocarbons, alkylbenzenes, polycyclic aromatic hydrocarbons, and their chlorinated derivatives, it has been confirmed that the partition mode and the size exclusion mode work exclusively for the elution of PCB and insulating oil, respectively, from PVA gel. It has also been observed that the elution position for each organic compound examined here varies roughly linearly with its inorganic value/organic value, used as an index in an organic conceptional diagram. Thus, PCB can be completely separated from insulating oil by its strong partition on PVA gel, because the index of the inorganic value/organic value of PCB is much higher than that of insulating oil. This separation mechanism is also explained well by using thermodynamic data (△H) estimated from the slope of a van't Hoff plot. As a result, the separation characteristic on PVA gel can be evaluated by using the index of the inorganic value/organic value as well as a thermodynamic data.