ローヤルゼリー中の残留キノロン剤分析のためのイムノアフィニティークロマトグラフィーを用いる前処理法

摘要

A sample preparation method was developed for the determination of qunolones in royal jerry (RJ) using immunoaffinity chromatography. For this purpose, an immunoaffinity column was prepared by packing a quinolone-specific monoclonal antibody immobilized to agarose resin. An RJ sample diluted with phosphate buffer was centrifuged. Then the supernatant was applied to the column. After the column was washed, quinolones were eluted with glycine-HCl. Quinolones in the eluate were determined by HPLC with fluorescence detection. No interfering peak was found on the chromatogram of the RJ sample. The recoveries of quinolones from the samples were over 75% at fortification levels of 25 ng/g for norfloxacin, 10 ng/g for dano-floxacin and 50 ng/g for enrofloxacin and ciprofloxacin. This sample-preprocessing method using immunoaffinity chromatography was found to be effective and suitable for determining residual quinolones in RJ.%キノロン剤は幅広い抗菌スペクトルを有する合成抗菌剤rnであり，ヒトのみならず，家畜の下痢症，肺炎など消化器rn系や呼吸器系の感染症及び魚類の腸炎，尾腐れ病など細菌rn性疾病の予防・治療薬として畜水産業界でも広く使用されrnている．食用に供される畜水産物については，休薬期間がrn遵守されていれば，抗菌剤が残留した食品が流通することrnはないはずであるが，検疫所や保健所など行政機関でのモrnニタリング検査や抜き取り検査において残留報告が散見さrnれることがある．