Transformation of Coat Protein Encoding Gene from Soil-Borne Mosaic Virus into Wheat

摘要

CWMV-CP1 target gene and bar selection gene were co-transferred into commercial wheat variety of Yangmai158 by particle bombardment. In total, 145 resistant plants to 3 - 5 mg L~(-1) Bialaphos were obtained , 21 plants were identified to be positive in T_0 generation by PCR-Southern test, and the transformation frequency had 0. 99%. T_1 plants were further tested by PCR and Southern hybridization. Results demonstrated that the alien resistance gene had been integrated into the wheat genome. The segregation ratio of CP1~+ to CP1~- in T_1 generation was 1. 0 to 1. 3, and didn't agree with Mendelian rule. RT-PCR result from T_2 plants showed that the alien gene CWMV-CP1 had stable expression in wheat genetic background.