Hepatic Lipogenesis Associated with Biochemical Changes in Overfed Landaise Geese and China Xupu Geese

摘要

This experiment studied hepatic lipogenesis associated with biochemical changes in overfed Landaise Geese and China Xupu geese. Twenty healthy male Landaise geese and 20 healthy male Xupu geese, hatched on the same day under the same feeding conditions, were selected as experimental animals. The animals were divided into two groups and each breed served as an experimental group. Per goose of per experimental group served for a repeat. Brown rice was selected as test diet. After overfeeding for 21 d and then slaughtering, the biochemical changes of hepatic lipogenesis in the genetic susceptibility to fatty liver were evaluated. These results showed that (1) the weight of fatty liver of the two breeds of geese were 801 and 375 g (P<0.05), respectively. There were no differences on the abdominal fat pat, filet total and filet pectoralis major in the two breeds experimental of the geese group (P<0.05) and no difference on body and filet skin plus subcutaneous adipose tissue (P>0.05) was found; (2) in these two breeds of geese, there were no differences on very-low-density lipoprotein (VLDL), cholesteryl esters (CE) (P<0.05), free cholesterol (FC), triglycerides (TG), phospholipids (PL) and protein (P<0.05); (3) there were no differences on activitiesof malic enzyme (ME), glucose-6-phosphatedehydrogenase (G6PDH), acetyl-CoA-carboxylas (ACX), fatty acid synthase (FAS), and mRNA level of ME in the two experimental breeds of geese groups (P<0.05); (4) test in Landaise geese group showed that there was no significant correlation with the specific enzymatic activities, while in Xupu geese group, the liver weight was negatively correlated to the specific activity of ACX and positively to that of ME; (5) in these overfed geese, ME activity appeared to be amajor factor involved in the genetic susceptibility to hepatic steatosis and it determined the hepatic lipogenesis capacity.