Quantification of airborne Aspergillus fumigatus spores in rabbit houses by real-time polymerase chain reaction

摘要

To accurately quantify airborne Aspergillus fumigatus (A. fumigatus) spores in rabbit houses, the real-time polymerase chain reaction (real-time PCR) and culture-based counting method (CCM) were employed to determine the airborne A. fumigatus spore concentrations. The results showed that, of the three rabbit houses (A, B, and C), the average concentrations of airborne A. fumigatus spores determined by real-time PCR were 3.0 × 10~3, 3.3 × 10~3, and 1.5 × 10~3 spores/m~3 air, respectively, while those determined by CCM were 2.5 × 10~2, 2.8 × 10~2, and 1.1 × 10~2 colony-forming unit/m~3 air (CFU/m~3 air), respectively, i.e., the former concentration was 12-14 times higher than the latter one. Therefore, the conventional CCM underestimated the concentrations of airborne fungal spores, and it is insufficient to determine the microbial aerosol concentration and evaluate the health risk only using CCM.