Studies on the Interactions between Pto and Ptils Proteins from Maize by a Yeast Two-Hybrid System

摘要

To check whether the protein ZmPto can interact with the protein ZmPtils, in this study, the gene sequences of ZmPto and ZmPtila, ZmPtilb were inserted into yeast expression vectors pEG202 and pJG4-5, respectively. Yeast two-hybrid was then conducted to verify their interactions. Results showed that yeast strains neither carrying empty plasmid nor carrying only one single fusion sequence of ZmPtil or ZmPto can activate reporter genes. Only the yeast strains carrying both fusion sequence of ZmPtil and ZmPto can activate reporter genes indicating that ZmPto can interact directly with ZmPtil in the yeast two-hybrid system.