Effects of advanced glycosylation end products on proliferation and cytosolic free calcium in cultured rat aortic smooth muscle cells

摘要

AIM: To study the effects of advanced glycosylation end products (AGEP) on aortic smooth muscle cell (ASMC) proliferation and its relationship with cytosolic free calcium ([Ca~2+]_i). METHODS: The effects of AGEP modified bovine serum albumin (AGEP-BSA) on the incorporation of [~3H]TdR and [~3H] Leu into cultured ASMC were observed. The [Ca~2+]_i of cultured ASMC was determined with Fura 2-AM. RESULTS: AGEP-BSA stimulated the incorporation of [~3H]TdR and [~3H]Leu into ASMC in concentration and time-dependent manners(P< 0.01), especially [~3H]TdR. In AGEP 200 mg·L~-1 group, after 12-h exposure, the incorporation of [~3H]TdR obviously increased, but DNA synthesis was concentration-dependently decreased in AGEP 300- 400 mg·L~-1 groups.