Effect of Coriaria lactone on cytosolic free calcium of cultured neurons from rat cerebral cortex

摘要

Conclusion: The [Ca~2+]_i of cultured neurons was elevated by CL, depending on extracellular Ca~2+. Aim: To study the effect of Coriaria lactone (CL)on cytosolic free calcium([Ca~2+]_i)of cultured neurons from cerebral cortex. Methods: primary neuron culture(14 d)and AR-CM-MIC cation measurement system were Used, the[Ca~2+]_I were measured. CL effect Was observed by loading egtazic acid. Results: The [Ca~2+]_i of cultured neurons (99.4-103.4)nmol.L~-1 was elevated concentration-dependently be CL(25-500) μmol.L~-1(P < 0.01).