High-throughput screening for human collagenase 1 inhibitors

摘要

The screening was accomplished within 2 h and 10 min with consumption of each com- pound at 4μg. Sixty-six compounds were identified with > 60/100 inhibitory activity at 20 mg/L, among which 44 compounds were confirmed by subsequent test- int at multiple concentrations. The most potent com- pound showed an IC_50 at 4.3μmol/L. The high-throughput method using the re- Combinant collagenase is fast, effective and practical in I- Dentifying inhibitors.