Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells

摘要

When the cells were treated with antisens VEGF ODN(6.25-50μmol.L~-1), VEGF mRNA level in the cells decreased remarkably in a concentration-dependent manner. No change was found when the cells were treated with sense or missense ODN. When the cells were treated with antisense VEGF ODN25 Μmol.L~-1, VEGF protein level decreased greatly both in The cells and the media. Antisense VEGF ODN inhibited VEGF expression specifically in A172 cells in vitro and thus the results provided the basis For the further studies in vivo.