Dynamic Combinatorial Chemistry to Identify Binders of ThiT an S‐Component of the Energy‐Coupling Factor Transporter for Thiamine

摘要

We applied dynamic combinatorial chemistry (DCC) to identify ligands of ThiT, the S‐component of the energy‐coupling factor (ECF) transporter for thiamine in Lactococcus lactis. We used a pre‐equilibrated dynamic combinatorial library (DCL) and saturation‐transfer difference (STD) NMR spectroscopy to identify ligands of ThiT. This is the first report in which DCC is used for fragment growing to an ill‐defined pocket, and one of the first reports for its application with an integral membrane protein as target.