Maximum tolerated doses and erythropoiesis effects in the mouse bone marrow by 79 pesticides’ technical materials assessed with the micronucleus assay

摘要

class="kwd-title">Abbreviations: CI, confidence interval of the mean; mg/kg b.w., milligram per kilogram of body weight; MTD, maximum tolerated dose; NCE, normochromatic erythrocyte; PCE, polychromatic erythrocyte; Es, total erythrocytes; MN test, micronucleus test class="kwd-title">Keywords: Pesticides, Triazoles, Maximum tolerated doses, Erythropoiesis, Micronucleus test, Mice class="head no_bottom_margin" id="abs0015title">AbstractEffects of technical materials of pesticide active ingredients, belonging to various chemical classes, on erythropoiesis in mouse bone marrow were studied as part of the research on the pesticide mutagenic activity in micronucleus test. The purpose of the present study was to estimate the toxic action of the test substances on the target organ and the validity of the results of the micronucleus assay under conditions of erythropoiesis suppression.It was demonstrated that intragastrically administrated triazole pesticides reached bone marrow (target organ where micronucleus induction was assessed) and exerted an inhibitory effect on erythropoiesis. The effects of triazole pesticides were enhanced in the following order: difenoconazole ≤ tebuconazole < cyproconazole < flutriafol. Furthermore, an association between structural features of molecules and specific target organ activity of the test pesticides was observed.Based on the data on the general toxicity and the results of the evaluation of the effects on erythropoiesis, the maximum tolerated doses (MTDs) of 79 different technical materials of pesticides for CD-1 mice were determined.