A Proteomic Approach for Comprehensively Screening Substrates of Protein Kinases Such as Rho-Kinase

摘要

BackgroundProtein kinases are major components of signal transduction pathways in multiple cellular processes. Kinases directly interact with and phosphorylate downstream substrates, thus modulating their functions. Despite the importance of identifying substrates in order to more fully understand the signaling network of respective kinases, efficient methods to search for substrates remain poorly explored.