Deep Sequencing of Target Linkage Assay-Identified Regions in Familial Breast Cancer: Methods Analysis Pipeline and Troubleshooting

摘要

BackgroundThe classical candidate-gene approach has failed to identify novel breast cancer susceptibility genes. Nowadays, massive parallel sequencing technology allows the development of studies unaffordable a few years ago. However, analysis protocols are not yet sufficiently developed to extract all information from the huge amount of data obtained.