2066. Accelerated Detection of Carbapenem Resistance Mechanisms in Enterobacteriaceae by MALDI-TOF Mass Spectrometry Using the Direct-on-Target Microdroplet Growth Assay (DOT-MGA)

摘要

BackgroundThe differential identification of carbapenemases relies mostly on molecular techniques. Current phenotypic methods require 18 hours of incubation. We propose a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based direct-on-target microdroplet growth assay (DOT-MGA) aiming to offer an easy and rapid phenotypic identification of AmpC, KPC, MBL and OXA production.