DIPG-14. MULTISITE STEREOTACTIC NEEDLE ASPIRATION BIOPSY IN PEDIATRIC DIFFUSE INTRINSIC PONTINE GLIOMA — A PROMISED SAFE PROCEDURE

摘要

BACKGROUND: Diffuse intrinsic pontine glioma (DIPG) is a highly malignant tumor raised from pons, causing various symptoms in affected cranial nerves. DIPG accounts for about 10% in tumors of central nervous system tumors in children. DIPG has a poor prognosis, with less than 10 percent of the children surviving for more than two years. OBJECTIVE: This study is a retrospective study in assessment of the safety of multisite stereotactic needle aspiration biopsy in children with DIPG. Furthermore, biological characteristics of DIPGs will be studied in advanced molecular pathology. METHOD: Clinical data of children with DIPG from December 2006 to July 2018 in Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine was collected and analyzed. Conditions of the patients in post stereotactic biopsy and follow-up period were compared with those with open biopsy. DNA methylation array (Illumina 850K) and H3K27M mutation measurement were conducted. RESULT: 66 patients with DIPG were enrolled with 35 males and 31 females. KPS score range of 50 to 80 points, the median was 60 points. 55 cases were positive in H3K27M test, 9 cases were negative in H3K27M test. The Kaplan-Meier analysis showed that there was no significant difference in survival by gender, biopsy methods and with/without chemotherapy. KPSs shifts were only found in 3 patients whose scores were lower than 40 before biopsy (either open or stereotactic biopsy). Different biopsy sites in multisite stereotactic biopsy showed different pathology characteristics in DIPG. CONCLUSION: DIPG is a deadly malignancy with poor prognosis. While, stereotactic biopsy is a promised safe procedure for patients and could provide valuable biological information for further research and target therapies.