Transplantation of Human Neuroblastoma Cells Catecholaminergic and Non-Catecholaminergic: Effects on Rotational Behavoir in Parkinsons Rat Model

摘要

Cultured human catecholaminergic and noncatecholaminergic donor cells were used in neural transplantation experiments in a rat model of Parkinson's disease. Using two different human catecholaminergic neuroblastoma cell lines, one control non-catecholaminergic neuroblastoma cell line, and one sham control (tissue culture medium), transplants were made into the striatum using a modified Ungerstedt hemiparkinsonian rat model. Significant decreases in apomorphine-induced rotational behavior were produced by two of three catecholaminergic cell lines. Grafted cells staining positively for tyrosine hydroxylase (TH) and catecholamine fluorescence indicated viable catecholamine activity in the two cell lines which produced reductions in rotational behavior.Catecholamine fluorescence was not detected ineither of the two controls. These data suggest alink between catecholamine secretion by transplantedcells and motor improvement using a ratrotational behavior model.