With pinacyanol as the supravital stain, a preferential effect on mitochondria of KB cells was achieved by the irradiation with the ruby laser beam. The observation confirmed the results of other workers using janus green B in the same experimental system. The preferential effect on mitochondria was noted in the area extending 8–10 µ beyond the nonpreferential damage of 4–5 µ in diameter. The opaque material associated with mitochondria possibly represented coagulated protein. The effect involved cristae mitochondriales without severe disarrangement of their structure. The opaque material could be interpreted as the result of direct interaction between mitochondria and the laser beam, even though the mitochondria were noted outside of the previously estimated focal spot size of about 3 µ Within the thickness of 2–4 µ of monolayered cells, larger areas of damage can be accounted for by divergence of the beam which is focused by a microscope objective of very short focal length. A threshold of biologic effectiveness is probably also involved.
展开▼
机译：用松果醇作为表面染色剂，通过红宝石激光束的照射，对KB细胞的线粒体具有优先作用。观察结果证实了其他工人在同一实验系统中使用janus green B的结果。线粒体的优先作用在超出直径4–5 µ的非优先损伤的8–10 µ的区域中可见。与线粒体相关的不透明物质可能代表了凝结的蛋白质。该作用涉及cr线粒体而没有严重破坏其结构。不透明的物质可以解释为线粒体和激光束之间直接相互作用的结果，即使注意到线粒体超出了先前估计的焦点尺寸约3 µ，在单层细胞2-4 µ的厚度范围内，较大损坏的区域可以通过光束的发散来解决，光束的发散由非常短焦距的显微镜物镜聚焦。生物有效性的阈值也可能涉及。
展开▼
