Computerized cyclic voltammetric detectionafter HPLC of the antineoplastic agentsetoposide teniposide adriamycin and itsmetabolite adriamycinol in urine samples

摘要

A computerized electrochemical detection system for application after HPLC, provided with a cyclic voltammetric oxidative and reductive module, is described for the on-line qualitative determination of electroactive antineoplastic agents and metabolites in urine samples, collected from cancer patients, following intravenous administration.The application of two cyclic voltammetric detection modes provides an insight into both oxidative and reductive electrode reactions of compounds, passing the detector and the occurrence of (it)reversible chemical and electrochemical processes at the electrode surface. In this way, redox properties of drugs and metabolites characteristic of their molecular structure, can be established, which may provide information related to their (enzymatic) bioactivation.In the cyclic voltammetric mode, the system permits automatic detection of a compound in the cell, recording, storage and plotting of voltammograms and calculation of the retention times, the half-wave potentials and the peak potentials of each scan of all individual compounds. For routine use, storage of 68 voltammograms on-line is sufficient for the analysis of biological samples inclinical-pharmacological research. Special attention has been paidto automatic, multi-reference-point component detection.Based on their concentrations in urine, the oxidative cyclicvoltammetric mode, using a glassy carbon electrode, permits thedetermination of etoposide and teniposide, whereas the reductivecyclic voltammetric mode, with a static mercury drop electrode,permits the determination of adriamycin and its metaboliteadriamycinol.