Immuno-Histochemical Studies of Connective Tissue: The Use of Contrasting Fluorescent Protein Tracers in the Comparison of two Antisera

摘要

Two fluorochromes of contrasting colour, fluorescein and lissamine rhodamine B200 have been used in an application of Coons's immuno-histochemical technique to a comparison of the reactions with their antigens in tissue sections of anti-human-glomerulus antisera and anti-human-synovium antisera.A uniform redistribution of labelled antibodies took place when tissue sections pre-treated with fluorescein labelled anti-glomerulus globulin were exposed to anti-glomerulus globulin labelled with lissamine rhodamine B200.In sections pre-treated with an anti-human-glomerulus conjugate and exposed to an anti-human-synovium conjugate labelled contrastingly, and vice versa, the redistribution of antibodies did not occur to the same extent in every situation.The findings can be explained by the hypothesis that there is a difference in the stability of union between certain connective tissue antigens and antibodies present in anti-glomerulus conjugates, on the one hand, and anti-synovium conjugates on the other.The results of these experiments and of others in which anti-glomerulus and anti-synovium conjugates labelled contrastingly were allowed to react with tissue sections simultaneously, indicate that splenic reticulin is antigenically dissimilar to fibrils in vascular adventitia.