As part of the , we published a Registered Report (Repass et al., 2016), that described how we intended to replicate an experiment from the paper ‘Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma’ (Castellarin et al., 2012). Here we report the results. When measuring Fusobacterium nucleatum DNA by qPCR in colorectal carcinoma (CRC), adjacent normal tissue, and separate matched control tissue, we did not detect a signal for F. nucleatum in most samples: 25% of CRCs, 15% of adjacent normal, and 0% of matched control tissue were positive based on quantitative PCR (qPCR) and confirmed by sequencing of the qPCR products. When only samples with detectable F. nucleatum in CRC and adjacent normal tissue were compared, the difference was not statistically significant, while the original study reported a statistically significant increase in F. nucleatum expression in CRC compared to adjacent normal tissue (Figure 2; Castellarin et al., 2012). Finally, we report a meta-analysis of the result, which suggests F. nucleatum expression is increased in CRC, but is confounded by the inability to detect F. nucleatum in most samples. The difference in F. nucleatum expression between CRC and adjacent normal tissues was thus smaller than the original study, and not detected in most samples.
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机译：作为的一部分，我们发布了注册报告（Repass等，2016），其中描述了我们打算如何复制论文“核仁融合杆菌在人类大肠癌中很普遍”的实验（Castellarin等，2012）。 。在这里，我们报告结果。通过qPCR在大肠癌（CRC），邻近正常组织和单独的匹配对照组织中通过核糖核酸测定DNA时，我们在大多数样品中均未检测到核镰刀菌信号：25％的CRC，15％的邻近正常细胞和基于定量PCR（qPCR），0％的匹配对照组织为阳性，并通过qPCR产物的测序确认。当仅比较CRC和邻近正常组织中可检测到的F. nucleatum的样品时，差异无统计学意义，而原始研究报道，与邻近正常组织相比CRC中CRC F. nucleatum表达有统计学意义的增加（图2； Castellarin等人，2012年）。最后，我们报告了该结果的荟萃分析，表明核仁中的核仁表达增加了，但由于无法检测到大多数样品中的核仁而感到困惑。因此，CRC与邻近正常组织之间的核镰刀菌表达差异小于原始研究，并且在大多数样品中未检测到。
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