Functionalized HydrophilicSuperparamagnetic IronOxide Nanoparticles for Magnetic Fluid Hyperthermia Application inLiver Cancer Treatment

摘要

In this work, we report the synthesis of hydrophilic and surface-functionalized superparamagnetic iron oxide nanoparticles (SPIOs) to utilize them as nanomedicines for treating liver cancer via magnetic fluid hyperthermia (MFH)-based thermotherapy. For this purpose, initially, we have synthesized the SPIOs through co-precipitation/thermolysis methods, followed by in situ surface functionalization with short-chained molecules, such as 1,4-diaminobenzene (14DAB), 4-aminobenzoic acid (4ABA) and 3,4-diaminobenzoic acid (34DABA) and their combination with terephthalic acid (TA)/2-aminoterephthalic acid (ATA)/trimesic acid (TMA)/pyromellitic acid (PMA) molecules. The as-prepared SPIOs are investigated for their structure, morphology, water dispersibility, and magnetic properties. The heating efficacies of the SPIOs are studied in calorimetric MFH (C-MFH) with respect to their concentrations, surface coatings, dispersion medium, and applied alternating magnetic fields (AMFs). Although all of the as-prepared SPIOs have exhibited superparamagnetic behavior, only 14DAB-, 4ABA-, 34DABA-, and 4ABA-TA-coated SPIOs have shown higher magnetizationvalues (Ms = 55–71 emu g^–1) and good water dispersibility. In C-MFH studies, 34DABA-coatedSPIO-based aqueous ferrofluid (AFF) has revealed faster thermal responseto the applied AMF and reached therapeutic temperature even at thelowest concentration (0.5 mg mL^–1) compared with14DAB-, 4ABA-, and 4ABA-TA-coated SPIO-based AFFs. Moreover, 34DABA-coatedSPIO-based AFF has exhibited high heating efficacies (i.e., specificabsorption rate/intrinsic loss power values of 432.1 W gFe^–1/5.2 nHm² kg^–1 at0.5 mg mL^–1), which could be mainly due to (i) enhancedπ–π conjugation paths of surface-attached 34DABAcoating molecules because of intrafunctional group attractions and(ii) improved anisotropy from the formation of clusters/linear chainsof the SPIOs in ferrofluid suspensions, owing to interfunctional groupattractions/interparticle interactions. Moreover, the 34DABA-coatedSPIOs have demonstrated (i) very good cytocompatibility for 24/48h incubation periods and (ii) higher killing efficiency of 61–88%(via MFH) in HepG2 liver cancer cells as compared to their treatmentwith only AMF/water-bath-based thermotherapy. In summary, the 34DABA-coatedSPIOs are very promising heat-inducing agents for MFH-based thermotherapyand thus could be used as effective nanomedicines for cancer treatments.